In stem cell research there are several key methods that, once mastered, can be extremely powerful. These methods enable you to rigorously test hypotheses, compare results to gold standards,ù and may even spur improvements to existing protocols. This book describes numerous methods to derive, manipulate, target, and prepare stem cells for clinical use. The methods described here help you: Derive and test human embryonic stem cells; Analyze bone marrow stem cell function in vitro and in vivo; Image a stem cell transplant; Cryopreserve stem cells; Differentiate stem cells using microscale techniques.
Preface ; Somatic Cell Nuclear Transfer and Derivation of Embryonic Stem Cells -Introduction. Materials for Nuclear Transfer. Methods for Nuclear Transfer. Derivation of Mouse ntES Cells. Materials for ES Cell Derivation. Methods for ES Cell Derivation. Discussion and Commentary. Summary Points. ; Derivation of Mouse Parthenogenetic Embryonic Stem Cells -Introduction. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Summary Points. ; Generation of Mice from Embryonic Stem Cells Using Tetraploid Embryos as Hosts -Introduction. Experimental Design. Materials. Methods. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points. ; Bioreactor Design and Implementation -Introduction. Experimental Methods and Materials. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points.; Extracellular Matrix Microarrays and Stem Cell Fate -Introduction. Experimental Design. Materials. Methods. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points. ; Microfluidic Culture Platform for Investigating the Proliferation and Differentiation of Stem Cells -Introduction. Experimental Design. Materials and Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Application Notes. Summary Points. ; Analysis of Mouse Hematopoietic Stem and Progenitor Cells -Introduction. Experimental Design of Lineage Depletion of Whole Bone Marrow Cells. Materials for Lineage Depletion of Whole Bone Marrow Cells. Methylcellulose-Based in Vitro Colony-Forming Assay. Radiation of Mice for In Vivo Assays. Colony-Forming Unit-Spleen Assay. Quantification of HSCs Using the Limiting Dilution Assay. Summary Points. ; Skeletal Stem Cells and the Hematopoietic Microenvironment: Biology and Assays -Introduction. Experimental Design. Materials. Method. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points. ; Targeting the Stem Cell Niche In Vivo -Introduction. Experimental Design. Materials. Methods. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points. ; Parabiosis in Aging Research and Regenerative Medicine -Introduction. Experimental Design for Aging Studies. Materials. Methods. Troubleshooting Parabiotic Disease. Discussion and Commentary. ; Utilization of the Mixed Lymphocyte Reaction Assay to Determine Stem Cell Immunogenicity and Suppression -Introduction. Experimental Design. Materials. Methods. Data Acquisition, Anticipated Results, Interpretation, and Statistical Guidelines. Discussion and Commentary. Application Notes. Summary Points. ; A Novel Method for the Preservation of Embryonic Stem Cells Using a Quartz Capillary Freezing System -Introduction. Experimental Design. Materials. Methods. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points. ; In Vivo MR Tracking of hESC-Derived Oligodendrocyte Precursors in Mouse Brain -Introduction. Experimental Design. Materials. Methods. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points. ; About the Editors. List of Contributors. Index ;
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Biju Parekkadan
Biju Parekkadan is an instructor in surgery and bioengineering at Massachusetts General Hospital. His research focus is in the area of stem cell bioengineering. He holds a B.S. in biomedical engineering from Rutgers University and a Ph.D. in chemical and medical engineering from the Harvard-MIT Division of Health Sciences and Technology.
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Martin Yarmush
Martin Yarmush is the Helen Andrus Benedict Professor of Surgery and Bioengineering at the Harvard Medical School and Director of the Center for Engineering in Medicine at Massachusetts General Hospital. Dr. Yarmush is an internationally recognized engineer and biophysical chemist. He is known as one of the leading investigators in the area of molecular and cellular bionengineering through seminal contributions to the fields of hepatic and skin tissue engineering, applied immunology, metabolic engineering, and geonomics and proteomics.