This practical book is part of the new Artech House Methods in Bioengineering series - volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. Written and edited by recognized experts in the field, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This unique volume covers in detail the basic techniques and protocols of regenerative medicine, focusing on methods for cell transplantation. You explore a wide range of critical factors, such as grown tissues, cells, laboratory-made compounds, and engineered organs. The books helps you understand the many intricate steps involved in tissue engineering, cell isolation, progenitors and stem cell differentiation, and in vivo models for assessing new strategies.
Extracellular Matrices for Hepatic Tissue Engineering -Introduction. Experimental Design. Materials. Methods. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points.; Tissue Engineering Using a Temperature-Responsive Cell Culture Dish -Introduction. Experimental Design. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Applications Notes. Summary Points.; In-Vivo Functional Assessment of Engineered Human Insulin-Producing Cells -Introduction. Experimental Design. Materials. Methods. Anticipated Results. Discussion and Commentary. Application Notes. Summary Points.; Generation of Insulin-Producing Cells from Adult Mouse Intrahepatic Biliary Epithelial Cells In Vitro -Introduction. Experimental Design. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Summary Points.; Technologies to Improve Human Islet Transplantation. -Introduction. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Summary Points.; Pancreatic Islet Isolation from Swine -Introduction. Materials. Equipment. Media. Methods. Anticipated Results. Discussion and Commentary. Summary.; Spinal Cord Injury Models and Cell Transplantation Therapy -Introduction. Experimental Design. Materials and Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Application Notes. Summary Points.; Cell Transplantation and Regeneration in Neurology -Introduction. Experimental Design. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Application Notes. Summary Points. Conclusions.; Biocompatible Genetic Modification of Multipotent Mesenchymal Stromal Cells -Introduction. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Application Notes. Summary Points. ; Adipose Tissue as a Source of Mesenchymal Stem Cells for Hepatocyte Transplantation -Introduction. Materials. Methods. Anticipated Results. Discussion and Commentary. Clinical Applications of Uncultured AT-MSCs. Summary Points. ; Culture Protocols for Producing Definitive Endoderm and Pancreatic Lineage Cells from Human ES or iPS Cells -Introduction. Experimental Design. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Application Notes. Summary Points.; Human Placenta Cell Isolation -Introduction. Placenta Embryological Development. Placenta Tissue Structure. Placenta Immunological Properties. Human Epithelial Cells from Amnion. Human Mesenchymal Stromal Cells from Amnion. Human Mesenchymal Stromal Cells from Chorion. Materials. Methods. Placenta-Isolated Cell Characterization. Cell Banking. Discussion and Commentary. Application Notes. Summary Points.; Liver Repopulation by Fetal Liver Stem/Progenitor Cells -Introduction. Experimental Design. Animals. Materials. Methods. Anticipated Results and Discussion. Summary Points.; Myoblast Transplantation: Techniques in Nonhuman Primates as a Bridge to Clinical Trials -Introduction. Experimental Design. Materials. Methods. Data Acquisition, Anticipated Results, and Interpretation. Discussion and Commentary. Application Notes. Summary Points.; Acronyms and Abbreviations. About the Editors. Contributors. Index.;
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Ira J. Fox
Ira J. Fox is the director of the Center for Regenerative Therapies at Children's Hospital of Pittsburgh and the MacGowan Institute for Regenerative Medicine. He is also a professor of surgery and pediatric transplantation at the University of Pittsburgh School of Medicine. He received his M.D. at Columbia University, New York.
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Nalu Navarro-Alvarez
Nalu Navarro-Alvarez is a research scientist in the Columbia Center for Translational Immunology (CCTI) at Columbia University. She earned her M.D. at the University of Guadalajara, Mexico and her Ph.D. at Okayama University Graduate School of Medicine and Dentistry, Japan.
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Alejandro Soto-Gutierrez
Alejandro Soto-Gutierrez is an assistant professor in the Department of Surgery, Transplantation Section at the Children's Hospital of Pittsburgh. Dr. Soto-Gutierrez earned his M.D. at the University of Guadalajara, Mexico and his Ph.D. at Okayama University Graduate School of Medicine and Dentistry, Japan.